Tsukuyomi will fail. It causes Overwhelming mental damage in the span of 2 seconds. It causes 0 physical damage. There's two ways hashi could possibly beat it;
1) He heals without seals, and considering tsunade was healing kakashi when he got tsuku stomped, hashi may be able to heal some of the critical mental exhaustion
2) Hashirama has massive stamina and a VERY strong life force (senju body; yang chakra; etc.) which means his mind would possibly be able to withstand the crippling, exhaustive damage of tsukuyomi.
3) least likel - since he has a massive chakra pool, he could possibly break tsuku; But, since only 2 seconds passes in the real world he most likely won't have enough time to rotate his chakra enough so that's very unlikely.
So? Featless tobirama and hiruzen aren't doing anything against hashi. Minato is his only good edo.