Well, I would say when we first got introduced to Sasuke's story, when Itachi died, when Sasuke learned the truth, when Naruto got beatten because of Sasuke (the summit of five kages), Jirayia's death, Naruto's talks (smart ones) definetely, Asuma's death (Shikamaru and his father's talk)...that would be it.
And I'm not ashamed to say that I cried for certain scenes.