Seeing such a simple and hardworking life being built by an old Pet Shop owner and his dog. Their love for eachother growing and their shop becoming a treasure cherished by both. Then the old man dying from his old age, but leaving the treasure to his dog, saying he will be back once it get's better...
It is sad to see that the dog keeps waiting for him, protecting the shop long after the man has gone. Knowing too well the man died, but still protecting the shop, for it is his treasure aswell.
And then seeing it be destroyed by a huge and powerfull pirate, a malicious monster. Fighting to protect it, even though he has no chance in beating it, until he is left bloody and beaten, crying to the roaring flames of it's shattered dreams...
And it's so heart-warming to see Luffy coming back with a small and ripped sack of pet food, all that remains from it's treasure, but with such great meaning behind it...
Also Chopper's story is soo moving. Being excluded from it's herd, just because he was born differently. Then eating a fruit that made him even weirder. Ostracized from it's family, looking for a meaning. He was the ugliest of two species, and couldn't find comfort in neither one. At such an young age, he couldn't figure out was HE did to them. The innocence of his question. Being excluded and hunted down blindly by others, and asking what he did wrong, as if he was to blame.
It was heart-warming that he found the doctor, and shared a deep relationship with him for a year. Refound his faith and his hapiness.
But then it turned so sad again, because the doctor was dying. Their relation was violently severed, using the weapon he hated and feared the most, later to find out that the reason why the doctor did it was because he was dying.
Then he decided to look for a cure. Inocent, sad, he believed that he could cure all the diseases in the world with one single miraculous medicine. The hardship he faced to find this one mushroom, so that he could cure the person he loved the most. A mushroom that bore the mark of the skull and the cross bone. His inocence associated the symbol to the opposite meaning, for that was what he was told. Everything the doctor said about the sympol was inspiring.
Can you imagine the pain he felt, knowing that the miraculous medicine he strove to find, was actually a deaddly poison? That he would be responsible for the death of his best and only friend?
It's so saaad
The last that I remember (I'm seeing the anime for the first time, so I haven't reach the the places I reached with the manga) was when Luffy declared war against the world, because the world declared war against Robin. My heart still sinks everytime I read anything about it