The last time she saw him was during Danzo's death/the run in with Kakashi, Naruto, and Sakura.
He asked for Itachi's eyes after this confrontation realizing he'd need them to defeat Naruto.
Just a guess, but I'd say there is probably a difference in his chakra after ascending to EMS.

Karin is still useful.
She is probably gonna be used to heal the kages.
Sasuke is useful...
Oh yeah, she'd love it. Not keen to see her bliss-out by healing Gaara though, the weirdo."Bite me Gaara!"
After escaping from the jail, Karin noticed Sasuke's chakra in the village and couldn't believe that it would be him.
i found this on wiki what does it mean?