yeeaaa..no.Chakra regenerates.At the moment that much chakra to be sealed in a baby was too much,so he had to cut it in half i suppose.
Chakra regenerates, yes. But this is a different case.
Normally chakra is transformed and used as jutsu, but in this case we are talking about Tailed Beasts and chakra being sealed. Minato ripped out half of Kurama's whole being and sealed it.
Let me make a couple of analogies:
You know how the Silver/Gold Brothers had some of Kurama's chakra. That chakra didn't regenerate to form a whole new Kurama.
Same thing with Sora, even though he had some of Kurama's chakra it didn't regenerate to form another Kurama.
I guess you could say chakra is like a bar.
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Normal usage of chakra just drains that bar, but it eventually recovers back to max chakra. But the huge sealing of chakra that Minato performed permanently decreases the max chakra.
We don't actually know. And just as it wasn't for Orochimaru, it's obvious that victims that survive the Dead Demon Seal don't get away from the Death god and can just regenerate what they lost. Until we're notified otherwise, we can't say it has its Yin chakra back. =/
Darn, that would've been easier to say than my post. Here's a cookie, +rep.