1 fingered Tsunade vs Konohamaru

[RedRobin]

The black lines on her body appear after the seal is released and the chakra begins flowing to repair any damage.

But again, with the network destroyed, the chakra can't flow anywhere. The technique can't be used.

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The markings dont need the network to move. They have chakra in them already. Whether her network is working or not they will still travel across her body. They carry chakra and activate the division which fixes the network since its cellular damage, the same level Byakugo works on.