I think that Black Zetsu could murder Hashirama Senju during the First Shinobi War.
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Overusing spirit is also a possibility...So many Hashi death threads lately.:lol I never really thought about how he died. Maybe we'll finally know in the Bolt series. BZ killing him could be a possibility. :hmm:
Could be the seal in his forehead looks similar to byakugou. He may also have used shinsuusenju which is a deity according to the databook. It gifted him with all nature releases. Like susanoo uses the user's lifeforce in return of using it' shinsuusenju might've taken hashi's life-force. But since hashi can go into sage mode and passively absorb energy like jugo, how'll there be ever a loss of body energy? I'll leave it up to theories.Hashirama probably died from rapid reproduction of his cells to regenerate, this shortening his lifespan.
He did tho.Nope he couldnt kill jj madara nor EMS madara. He just activated Kaguya , and he used obitos rikudou body to do that. He didnt kill hashirama , no way in hell.