Though i doubt madara has Tsukuyomi but he could have used normal sharingan genjutsu that he used against Ay etc. But he never even attempted to use genjutsu against hashirama probably because he likes to dance with him and fight power with power...! After all madara likes to feel the battle...
So Hashirama, the strongest Senju who fought Madara and other genjutsu-wielding Uchiha day in and day out for his whole life, is going to get caught by genjutsu?