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I think it is a misconception that any Senju or Uchiha who acquires the DNA of one another could simply awaken the Rinnegan with the same probability like Madara, this is false, the Rinnegan is awakened by the most optimal combination of the two dominant genetic traits of the original Rinnegan bearer: Rikudou Sennin
We know that both the Elder and Younger Son, each inherited a half of their Lord Father's genetic traits: The Powerful Body (Yang) and The Powerful Eyes (Yin) each of them received the optimal quality of those genetic traits in respect, meaning that among the Uchihas and Senjus, the Elder Son received the most potent quality of Rikudou's eyes while the Younger Son received the most potent genetic quality of Rikudou's body.
We know for a fact that neither the Uchiha nor the Senju ancestor possessed the Rinnegan, it is because each of them only received a half of their father's full genetic composition: the combination of the Eyes(Yin) and the Body(Yang)
Since Madara Uchiha discovered that combining the two dominant genetic traits of each ancestor would result in the awakening of the Rinnegan as an artificial process of reverse engineering the original Rinnegan (that of Rikudou's) then it only naturally follows that Madara would want the optimal genetic quality of these two dominant genetic traits as an emulation of the progenitors of the two clans (Uchiha and Senju)
Since Madara possessed the most powerful Sharingan (EMS) then he is the closest emulation of his ancestor (Elder Son)
so to assure the efficacy of the awakening of the Rinnegan, then it only follows that Madara must use the DNA of the most perfect emulation of the Younger Son's dominant genetic trait: that which is found in the person of the most powerful Senju: Hashirama
Through the combination of the DNA of the two most perfect emulation of each of their respective clans' ancestor, the optimal quality of the dominant genetic traits of the original bearer of the Rinnegan is achieved: that of Rikudou Sennin's
With these, the highest probability of awakening the Rinnegan is achieved, if Madara chose any lesser Senju then the reverse engineering of the original Rinnegan would have much lower chances and would have probably ended up in failure.
Madara (Representing the Uchiha Ancestor) and Hashirama (Representing the Senju Ancestor)
UPDATE
Clarifications
It is not only Hashirama's DNA but any Senju DNA combined with Uchiha DNA is capable of awakening the Rinnegan, so it is not a conditio sine qua non that only Hashirama's cells could be used to awaken the Rinnegan, however, Madara's choice in choosing to use Hashirama Senju's DNA is not a matter of necessity, but a matter of favorable outcome in terms of maximum probability, Madara, who has the most powerful Uchiha DNA would naturally chose the most powerful DNA of the Senjus: Hashirama's, since the combination of the optimal dominant genetic traits of these two bloodline woulds result in the maximum probability of awakening the Rinnegan, if Madara had chosen a less powerful Senju then his chances of awakening the Rinnegan decreases none the less, the mechanistic principle as specified in Manga canon remains, that Senju DNA per se combined with Uchiha DNA is possible in awakening the Rinnegan, but with lower chances of actualizing the desired outcome.
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We know that both the Elder and Younger Son, each inherited a half of their Lord Father's genetic traits: The Powerful Body (Yang) and The Powerful Eyes (Yin) each of them received the optimal quality of those genetic traits in respect, meaning that among the Uchihas and Senjus, the Elder Son received the most potent quality of Rikudou's eyes while the Younger Son received the most potent genetic quality of Rikudou's body.
You must be registered for see images
We know for a fact that neither the Uchiha nor the Senju ancestor possessed the Rinnegan, it is because each of them only received a half of their father's full genetic composition: the combination of the Eyes(Yin) and the Body(Yang)
Since Madara Uchiha discovered that combining the two dominant genetic traits of each ancestor would result in the awakening of the Rinnegan as an artificial process of reverse engineering the original Rinnegan (that of Rikudou's) then it only naturally follows that Madara would want the optimal genetic quality of these two dominant genetic traits as an emulation of the progenitors of the two clans (Uchiha and Senju)
You must be registered for see images
Since Madara possessed the most powerful Sharingan (EMS) then he is the closest emulation of his ancestor (Elder Son)
so to assure the efficacy of the awakening of the Rinnegan, then it only follows that Madara must use the DNA of the most perfect emulation of the Younger Son's dominant genetic trait: that which is found in the person of the most powerful Senju: Hashirama
You must be registered for see images
Through the combination of the DNA of the two most perfect emulation of each of their respective clans' ancestor, the optimal quality of the dominant genetic traits of the original bearer of the Rinnegan is achieved: that of Rikudou Sennin's
You must be registered for see images
With these, the highest probability of awakening the Rinnegan is achieved, if Madara chose any lesser Senju then the reverse engineering of the original Rinnegan would have much lower chances and would have probably ended up in failure.
Madara (Representing the Uchiha Ancestor) and Hashirama (Representing the Senju Ancestor)
You must be registered for see images
UPDATE
Clarifications
It is not only Hashirama's DNA but any Senju DNA combined with Uchiha DNA is capable of awakening the Rinnegan, so it is not a conditio sine qua non that only Hashirama's cells could be used to awaken the Rinnegan, however, Madara's choice in choosing to use Hashirama Senju's DNA is not a matter of necessity, but a matter of favorable outcome in terms of maximum probability, Madara, who has the most powerful Uchiha DNA would naturally chose the most powerful DNA of the Senjus: Hashirama's, since the combination of the optimal dominant genetic traits of these two bloodline woulds result in the maximum probability of awakening the Rinnegan, if Madara had chosen a less powerful Senju then his chances of awakening the Rinnegan decreases none the less, the mechanistic principle as specified in Manga canon remains, that Senju DNA per se combined with Uchiha DNA is possible in awakening the Rinnegan, but with lower chances of actualizing the desired outcome.
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