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Tsunade's Yin release(spirit/chakra) is applied with Tsunade's Yang (Body) to perform Creation Rebirth: resulting in the creation of new cells in the body.
This is how most understands it, however, sadly it is the wrong way of understanding. As I mentioned earlier, Kishi is keen on details. I think he knows a lot about human anatomy, and thus he would never do something like this. First of all it is important to note, that Tsunade does not create anything in her body. She takes advantage of her knowledge, of the mechanisms that lead to creation of new cells by cell division. This happens over and over in the body, however, it does not happen at a speed, in which it is possible to heal injuries on vital organs.
What Tsunade does, is that she simply speeds up the process of cell division, and thus she can heal the organs. She creates no new cells. She simply amplify the proteins in the cells with chakra, so that they can create the cell division even faster. No yin/yang hocus pocus. The only "yin" there is in it, is the sealing technique and the mechanisms involved with creating it, confining it, and releasing it.
Give or take, I found it in the manga now, it's just as I said U_U
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There is absolutely no yin or yang in it, and it has absolutely no relation to the SO6P's creation of all things.
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