During the fight with Madara's Perfect Susanoo, was Tsunade mysterious handseal..

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Two Byakugou users were required to summon 1/10 of Katsuyu's full body, so I doubt that.
hmm.. true, but i don't know how much chakra Tsunade has currently.. maybe Sakura is there to fill out the rest of chakra needed to summon the 1/10 Katsuyu.. though, honestly, i don't know how much chakra is needed to summon Katsuyu in her entirety, though it maybe gargantuan.. though a very weakened and near death Tsunade still had enough chakra to summon a mid size version of Katsuyu's standardized size..
 

TheEvilOne

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hmm.. true, but i don't know how much chakra Tsunade has currently.. maybe Sakura is there to fill out the rest of chakra needed to summon the 1/10 Katsuyu.. though, honestly, i don't know how much chakra is needed to summon Katsuyu in her entirety, though it maybe gargantuan.. though a very weakened and near death Tsunade still had enough chakra to summon a mid size version of Katsuyu's standardized size..
Well logically a rested Tsunade should have enough chakra to summon at least 5/10 of Katsuyu's original body. But probably she can't release so much chakra in a moment or something like this.
 

johnny335704

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It seems like - Tsunade was trying to release more chakra to prepare her moves to fight against Madara. Just a guess.
But it doesn't look like preparation for summoning.
 

Goddess Tsunade

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after knowing that Katsuyu is a damn huge thing,
I also thought that the hand seal she performed back then was to summon her in a bigger version to counter the PS
 
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