The massacre hadn't happened yet so I doubt it, and he'd still have to implant the eye before he could use it. Danzo was getting blitzed bullied by Sasuke so there's not much chance he was gonna be able to do much better against someone famous for his speed, with no Izanagi. One eyed Shisui is still to much for Danzo to handleOr shishui would have been slaughtered. Danzo's entire right arm is an izanagi museum. He already had the bandages on them at that point; they were clearly izanagi fest. Ontop of that, Shishui's own eye was already in his Socket. He could use shishui's own KA on shishui by that point.